Rich production media as a platform for CHO cell line development
نویسندگان
چکیده
منابع مشابه
High performance CHO cell line development platform for enhanced production of recombinant proteins including difficult-to-express proteins
Background In an effort to improve product yield of mammalian cell lines, we have previously demonstrated that our proprietary DNA elements, Selexis Genetic Elements (SGEs), increase the transcription of a given transgene, thus boosting the overall expression of a therapeutic protein drug in mammalian cells [1]. However, there are additional cellular bottlenecks, notably in the molecular machin...
متن کاملEstablishment a CHO Cell Line Expressing Human CD52 Molecule
Background: CD52 is a small glycoprotein with a GPI anchor at its C-terminus. CD52 is expressed by Normal and malignant T and B lymphocytes and monocytes. There are detectable amounts of soluble CD52 in plasma of patients with CLL and could be used as a tumor marker. Although the biological function of CD52 is unknown but it seems that CD52 may be involved in migration and activation of T-cells...
متن کاملMetabolic engineering of CHO cells for the development of a robust protein production platform
Chinese hamster ovary (CHO) cells are the most preferred mammalian host used for the bio-pharmaceutical production. A major challenge in metabolic engineering is to balance the flux of the tuned heterogonous metabolic pathway and achieve efficient metabolic response in a mammalian cellular system. Pyruvate carboxylase is an important network element for the cytoplasmic and mitochondrial metabol...
متن کاملDevelopment and evaluation of a new, specially tailored CHO media platform
Background Today’s biopharmaceutical industry is under increasing pressure considering cost efficient development. Short timeframes rule the progress starting from the generation of producer cell lines to the establishment of a final production process. Hence, the timescale for optimization of cell culture media is small, but on the other hand it contains high potential for global process impro...
متن کاملA platform for Chinese hamster ovary ( CHO ) cell genome engineering
.....................................................................................................................................4 1.0 Introduction..........................................................................................................................5 1.1 Expression systems for bio-manufacturing......................................................................5 1.2 CH...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: AMB Express
سال: 2020
ISSN: 2191-0855
DOI: 10.1186/s13568-020-01025-3